Molecular biology of rotaviruses. III. Isolation and characterization of temperature-sensitive mutants of bovine rotavirus
نویسندگان
چکیده
منابع مشابه
Molecular Characterization and Phylogenetic Analysis of the G6 and G10 Genotypes of Bovine Rotaviruses from Iran
Background and Aims: Rotaviruses are the main cause of gastroenteritis in the young of human and a variety of animals in the world. Rotavirus G6 and G10 are the most prevalent genotypes in cattle worldwide, but are also found in human with possible origination from animals. Materials and Methods: In this work, we have detected the G6 and G10 genotypes of bovine rotaviruses isolated from di...
متن کاملmolecular characterization of bovine rotaviruses in pakistan
conclusions bovine rotavirus presents 6% of calf diarrhea in pakistani cattle and buffalo samples. the vp4 and vp6 genes show closed relationships with bovine rotavirus reported from indian isolates. background group a bovine rotaviruses (brv) are one of the main factors of neonatal calf diarrhea and mortality around the world. the current study was carried out to assess the genetics of brv cir...
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One hundred and twenty-one sensitive (ts) sex-linked lethals were screened by means of X-ray-induced somatic crossing over to determine if any were ts cell-lethal mutants. Cell-lethal mutations were identified by their ability to block the development of homozygous clones when raised under restrictive conditions (29degrees). Twenty-two ts cell-lethal mutants were isolated and categorized into t...
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Ten temperature-sensitive mutants of Sendai virus, a paramyxovirus, were isolated and partially characterized. The mutants replicated in chicken embryo lung cells at 30 C, but not at 38 C; wild-type virus grew equally well at both temperatures. Complementation tests divided the mutants into seven groups. Six groups synthesized neither infectious virus nor RNA when incubated at 38 C from the beg...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1982
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.42.2.669-677.1982